Bacteriological and molecular characterization of Mycobacterium bovis isolates from tuberculous lesions collected among slaughtered cattle, Northwest Ethiopia.

BACKGROUND: In Ethiopia, the distribution of bovine tuberculosis (BTB) has long been known and documented as a major problem of animal health. However, the burden of circulating M. bovis strains is poorly understood in the country. Therefore; this study aimed to identify and characterize the mycobacterial isolates responsible for BTB in Northwest Ethiopia. METHODS: A cross-sectional study was conducted on tuberculous lesions that had been collected from slaughtered cattle between September 2018 to June 2019. Collected lesions were cultured and tested for tuberculous bacilli. The MPT64 assay and Genotype line probe assay (LPA) were used for identification of mycobacterial isolates, and region of deletion 4 (RD4) typing and spoligotyping were used to characterize the M. bovis strains. RESULTS: Of the total 1458 examined slaughtered cattle, only 62 (4.3, 95%CI; 0.0328-0.0542) had tuberculous lesions. The highest number of gross tuberculous lesions were observed from the lymph nodes of the thoracic cavity; at the mediastinal (40.3%, 25/62) and bronchial (22.6%, 14/62) lymph nodes. Of the 62 collected tuberculous lesions; 18 (29.0%) were culture positive for mycobacterium isolates, and only five isolates were confirmed for M. tuberculosis complex (MTBc) by the MPT64 assay and LPA. All the five MTBc isolates were positive for RD4 typing of M. bovis with a PCR product size of 446 bp, and no isolate was noticed to have M. tuberculosis. The detected M. bovis strains displayed five spoligotypes; with the common SB1176 and SB0133 M. bovis strains, although the two spoligotypes had not been previously reported. CONCLUSION: The present study shows that BTB in North Gondar, Ethiopia, is caused by M. bovis strains SB1176 and SB0033, with low frequency. Thus, the finding highlights the importance of continuous surveillance for mycobacterial strains in cattle populations.

Prevalence, Isolation, Identification, and Risk Factors of Major Bacterial Cause of Camel Subclinical Mastitis.

OBJECTIVE: A crosssectional study was conducted between September 2015 and August 2016 in the district of Afar Regional State, Northeastern Ethiopia, to characterize the most prevalent bacterial pathogens and identify the associated risk factors of camel subclinical mastitis. California mastitis test (CMT) was used as a screening test, and standard bacteriological methods were carried out for isolation and identification of the pathogens. RESULTS: Among the total 96 lactating camels examined, 25 were found positive with the overall prevalence of 26%, with 25% and 1% subclinical and clinical mastitis cases, respectively. Totally, 384 quarters of udder were examined; of these, 10 of them were blind while the rest 374 were nonblind teats. The quarter level prevalence of subclinical mastitis was 8.9%. The analysis showed that statistically significant difference (P < 0.05) of tick infestation and subclinical mastitis. Additionally, among the bacteriologically tested 34 CMT positive milk samples, all of them showed growth on nutrient and blood agar plate. Out of these culture isolates, the major bacterial pathogens identified were Staphylococcus aureus (8.7%), Staphylococcus hyicus (6.52%), Staphylococcus intermedius (6.52), Coagulase-negative staphylococci (19.57%), Bacillus (19.57%), Escherichia coli (6.52%), and Pasteurella multocida (6.52%) species. Therefore, appropriate control measures and awareness creation to the community should be practiced.

Household Practice Related to Zoonotic Diseases Transmission in Rural Community of Gondar Zuria District.

PURPOSE: The study was done from October 2019 to April 2020 for the purpose of identifying practices and characterizing the general public knowledge, attitudes and associated risk factors related to animal contact that influence zoonotic disease transmission in the rural household heads of North Gondar area, Amhara Regional State, Ethiopia. PATIENTS AND METHODS: During this study, a cross-sectional type of study was designed and a simple random sampling method was used to choose kebeles. Six kebeles were randomly selected using a lottery method. Systematic random sampling method was employed to select and question 65 household heads from each of the kebeles. In total, 390 structured questionnaires were prepared for those household heads and the data were collected using face to face interviews. Finally, the recorded data were examined using STATA version 16 statistical software and the frequency distribution of both variables was observed using descriptive statistics. Linear regression model was used to see the relation between household practices and the explanatory factors. RESULTS: The results showed that there was a gender difference in responsibility for managing livestock and the central aim of rearing livestock was for sale. Among 390 members of households, 38.7% understand a probability of disease transmission in their community between livestock, humans and wildlife, regardless of the fact that household practices related to zoonosis transmission were common. Of the households, 52.3% responded that they permit animals to go into kitchen and sleeping areas (95% CI = 1.15-2.73; P = 0.009). The factors which influence the household practices were agro-ecology activities; livestock management; number of individuals in the household; types of livestock reared; and zoonosis awareness. CONCLUSION: In general, the present study showed that the public had a very low awareness about major zoonotic diseases. This indicates the need for awareness creation through education and an inter-disciplinary health approach with close collaboration among veterinarians, public health practitioners and policymakers.